Generator

Part:BBa_K1333316:Design

Designed by: Wei Tang   Group: iGEM14_SYSU-China   (2014-09-24)


I13453-RBS-PⅢ(M13 phage)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We expression PIII under the regulation of pBAD induced by Ara, and this part is for the rescure experiment.


Source

PIII is from genomic sequence of M13 phage and I13453 is from previous part.

References